Fig. 2.

Myofiber typing and diameter of vastus lateralis muscle. Data of young males (n = 13), old males (n = 21), young females (n = 12), and old females (n = 20) are shown. (A) Representative pictures of immunofluorescence staining used to discriminate type 1 and type 2 myofibers (20× magnification). Pictures from left to right show staining of Myosin Heavy Chain 7 (type 1 myofibers, green), Dystrophin (sarcolemma, red), nuclei (DAPI in blue), and the merge of all channels. (B) Minimum Feret’s diameter of type 1 and (C) type 2 myofibers. (D) Percentage of type 1 and 2 myofibers. (E) Correlation between MYH7 expression (RNA-seq derived data) and proportion of type 1 myofibers. Values represent mean ± SEM, *p < 0.05, **p < 0.01, and ***p < 0.001