TABLE 6.
General characteristics of purified sucrose isomerasesa
| Organism | Maximum yield (%)b
|
Optimal temp (temp range, °C) | Optimal pH (pH range) | Km (mM) | Vmax (sp act) (U mg−1) | Reference | |
|---|---|---|---|---|---|---|---|
| Isomaltulose | Trehalulose | ||||||
| P. dispersa UQ68J | 91 | 3 | 30-35 (10-55) | 5 (3-8) | 40 | 638 (562) | This study |
| K. planticola UQ14S | 66 | 15 | 35 (10-60) | 6 (4-7) | 76 | 423 (351) | This study |
| Klebsiella sp. strain LX3 | 83 | 21 | 35 (15-40) | 6 (5-6.5) | 55 | NS (328)c | 30 |
| Klebsiella sp. | 86 | NS | 35 | 6.0-6.5 | 120 | 110 (NS) | 16 |
| S. plymuthica ATCC 15928 | 73 | 9 | 30 | 6.2 | 65 | 120 (NS) | 24 |
| E. rhapontici NCPPB 1578 | 85 | 15 | 30 | 7.0 | 280 | NS (4) | 2 |
| P. rubrum CBS574.77d | 86 | 9 | 30 | 5.5 | 140 | NS (NS) | 15 |
| P. mesoacidophila MX-45 | 8 | 91 | 40 (20-40) | 5.8 (4-8) | 19 | NS (14) | 12 |
| Bemisia argentifolii | 0 | NS | NS | 6 | 110 | NS (245) | 18 |
a
The results are the results for the isomaltulose product, except for MX-45 and Bemisia (trehalulose product).
b
Ultimate percentage of conversion of sucrose into isomaltulose or trehalulose.
c
NS, not specified.
d
Immobilized cells (not purified enzyme) were used to obtain the data for P. rubrum CBS574.77.