FIG. 3.
Autoradiograms of the products formed by using [14C]benzene (A), [14C]toluene (B), and [14C]ethylbenzene (C) by wild-type, chimeric, and mutant ISPBEDα subunits combined with the ISPBEDβ subunit and coexpressed ISPBEDα-ISPBEDβ, ISPTODα-ISPBEDβ, and ISPBODα-ISPBEDβ in the dioxygenase assay. The Rf values of the radiolabeled products are indicated on the left. The numbers on the autoradiograms indicate the percentages of the chain monohydroxylated and cis-diol products. The values are averages of two determinations. A repeat experiment gave similar results. Details of the experiment are described in Materials and Methods.