TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics |
|---|---|
| Strains | |
| E. coli JM109a | endA1 recA1 gyrA96 thi hsdR17 relA1 supE44 Δ(lac-proAB) [F′ traD36 proAB lacIqZΔM15] |
| E. coli CJ236b | dut-1 ung-1 thi-1 rela1 F′ pCJ105 (Cmr) |
| Plasmids | |
| pGEM 3Zf(+) | Plac lacZ Ampr |
| pKK223-3 | Plac Ampr |
| pCB1c | 1.6-kb DraI/EcoRI bedC1 insert in pGEM 3Zf(+) |
| pJRM502c | 2-kb EcoRI/Pst1 bedC1C2 insert in pKK223-3 |
| pJRM502-1 | 2-kb EcoRI/PstI todC1-bedC2 insert in pKK223-3 |
| pJRM502-2 | 2-kb EcoRI/PstI bodC1-bedC2 insert in pKK223-3 |
| pJRM503d | 1.7-kb EcoRI bedBA insert in pKK223-3 |
| pJRM504c | 1.6-kb EcoRI/HindIII bedC1 insert in pKK223-3 |
| pJRM505d | 0.7-kb EcoRI/PstI bedC2 insert in pKK223-3 |
| pJRM711 | 0.66-kb EcoRI todC2 insert in pKK223-3 |
| pSA109d | 1.6-kb EcoRI/PstI todC1 insert in pKK223-3 |
| pJRM5042 | 1.6-kb EcoRI/PstI todC1 modified insert in pKK223-3 |
| pJRM5041 | 1.6-kb EcoRI/PstI bodC1 insert in pKK223-3 |
| pJRM5041-1 | 1.6-kb EcoRI/HindIII bod2C1 insert in pKK223-3 |
| pJRM5041-2 | 1.6-kb EcoRI/HindIII bod3C1 insert in pKK223-3 |
| pCMB101 | 1.6-kb EcoRI/PstI tedC1 insert in pKK223-3 |
| pJRM5043 | 1.6-kb EcoRI/HindIII tedC1 modified insert in pKK223-3 |
| pCB1M1 | pCB1 (L285W) |
| pCB1M2 | pCB1 (A291S) |
| pCB1M3 | pCB1 (L285W/A291S) |
| pCB1M5 | pCB1 (V324I/I327V) |
| pCB1M7 | pCB1 (G404D) |
| pCB1M9 | pCB1 (K436R) |
| pJRM504M1 | pJRM504(L285W) |
| pJRM504M2 | pJRM504(A291S) |
| pJRM504M3 | pJRM504(L285W/A291S) |
| pJRM504M4e | pJRM504(I301V/T305S/I307L/L309V) |
| pJRM504M5 | pJRM504(V324I/I327V) |
| pJRM504M6e | pJRM504(L285W/A291S/G404D) |
| pJRM504M7 | pJRM504(G404D) |
| pJRM504M8e | pJRM504(I412V) |
| pJRM504M9 | pJRM504(K436R) |
| pJRM504M10e | pJRM504(E444D) |
a
See reference 54.
b
Obtained from Bio-Rad.
c
Gift of C. S. Butler.
d
Gift of S. Anguravirutt.
e
Mutant ISPBEDα generated by PCR-based mutagenesis.