TABLE 5.
Percentages of products formed by dioxygenase-catalyzed reactions with ISPBEDα, ISPTODα, or ISPBEDα(I301V/T305S/I307L/L309V) combined with the ISPBED β subunit by using benzene, toluene, or ethylbenzene as the substrate
| Substrate | Product | % of product formeda
|
||
|---|---|---|---|---|
| ISPBEDα | ISPTODα | ISPBEDα mutant | ||
| Benzene | Benzene cis-dihydrodiol | 46 | 37 | 42 |
| Phenolb | 35 | 30 | 37 | |
| Catecholb | 19 | 33 | 21 | |
| Toluene | Toluene cis-dihydrodiol | 3 | 45 | 12 |
| Benzaldehyde | 18 | 1 | 8 | |
| Benzyl alcohol | 53 | 6 | 31 | |
| o-Cresolb | 22 | 38 | 42 | |
| m-Cresolb | 4 | 10 | 7 | |
| Ethylbenzene | Ethylbenzene cis-dihydrodiol | 5 | 24 | 16 |
| 1-Phenethyl alcohol | 24 | 39 | 5 | |
| 2-Hydroxyethylbenzeneb | 58 | 30 | 65 | |
| 3-Hydroxyethylbenzeneb | 13 | 7 | 14 | |
a
The values are averages of two determinations. A repeat experiment gave similar results.
b
Product expected from thermal decomposition. See text for details.