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Increased granaticin production conferred by 2A7 results in increased antibacterial activity. S. lividans Δact Δred/2A7 containing cosmid 2A7 or the corresponding empty shuttle vector (pOSI700), combined with pSGran or the corresponding empty shuttle vector (pMBD14), were grown for 9 days and extracted (see the text). Extracts were used undiluted and diluted 10-, 100-, and 1,000-fold and tested for antibacterial activity.
