TABLE 3.
16S rRNA gene-targeted primers
| Short namea | Full nameb | Annealing temp (°C) | Sequence 5′-3′ | Specificityc | Reference |
|---|---|---|---|---|---|
| 616V | S-D-Bact-0008-a-S-18 | 52 | AGA GTT TGA TYM TGG CTC | Most Bacteria | 28 |
| 630R | S-D-Bact-1529-a-A-17 | 52 | CAK AAA GGA GGT GAT CC | Most Bacteria | 28 |
| 1492R | S-*-Proka-1492-a-A-19 | 52, 60c | GGY TAC CTT GTT ACG ACT T | Most Bacteria and Archaea | Modified from 29 |
| AT+94Fd | S-*-AT-0094-a-A-18 | 60 | GCC GGC GAG TGG CGA ACG | Genera Azoarcus, Thauera, and other bacteria | This study |
| ATD1420Rd | S-*AT-1420-a-S-20 | 60 | CCT ACT TCT GGT GAA ACC CA | Genera Azoarcus, Thauera, and Denitromonas | This study |
| RHC175Fe | S-*-Rhc-0175-a-A-19 | 60 | CCG CAT ATT CTG TGA GCA G | Genera Candidatus “Accumulibacter,” Rhodocyclus, Propionivibrio, Dechloromonas, Azospira, and Ferribacterium | This study |
| RHC+1289Re | S-*-Rhc-1289-a-S-18 | 60 | TCC GGA CTA CGA TCG GCT | Genera Candidatus “Accumulibacter,” Rhodocyclus, Propionivibrio, Dechloromonas, Azospira, Ferribacterium, and other bacteria | This study |
| ZOGLO66Ff | S-*-Zoglo-0066-a-A-18 | 60 | ACG GTA ACA GGG AGC TTG | Genus Zoogloea, not Z. resiniphila | This study |
| ZOGLO1421Rf | S-*Zoglo-1421-a-S-19 | 60 | CCT ACT TCT GGT AAA CCC C | Genus Zoogloea | This study |
a
The short name used in the reference or in this study.
b
The full name of the 16S rRNA gene-targeted oligonucleotide primer is based on the nomenclature of Alm et al. (2).
c
Target organisms with a perfectly matched primer target site.
d
Primer pair A.
e
Primer pair R.
f
Primer pair Z.