TABLE 1.
L. lactis strains and plasmids used in this work
| Strain or plasmid | Characteristics | Reference(s) or source |
|---|---|---|
| Strains | ||
| NZ9000 | MG1363 pepN::hisRK | 19 |
| NZ9010 | NZ9000 ldh::ery; Eryr | 3, 14 |
| HWA217 | Reduced phosphofructokinase activity | 1 |
| NZ9000(pNZ8148) | NZ9000 containing pNZ8148 plasmid | This work |
| NZ9000(pWW002) | NZ9000 containing pWW002 plasmid | This work |
| NZ9010(pWW002) | NZ9010 containing pWW002 plasmid | This work |
| HWA217(pWW002) | HWA217 containing pWW002 and pNZ9530 plasmids | This work |
| NZ9000(pWW003) | NZ9000 containing pWW003 plasmid | This work |
| NZ9010(pWW003) | NZ9010 containing pWW003 plasmid | This work |
| HWA217(pWW003) | HWA217 containing pWW003 and pNZ9530 plasmids | This work |
| Plasmids | ||
| PQE60-M1Pase | E. coli expression vector pQE60 carrying the E. tenella M1Pase gene | 20 |
| pNZ8148 | pNZ8048 derivative; Cmr, lactococcal cloning and expression vector with nisA promoter upstream of a multiple cloning site | 19 |
| pNZ9530 | Eryr, hisRK | 17 |
| pNZ-mtlD | pNZ8148 carrying L. plantarum mtlD gene fused to nisA promoter | 30 |
| pWW002 | pNZ8148 carrying E. tenella M1Pase gene fused to nisA promoter | This work |
| pWW003 | pNZ-mtlD with E. tenella M1Pase gene cloned downstream of mtlD | This work |