TABLE 3.
Product formation by L. lactis strains overproducing E. tenella M1Pase and L. plantarum M1PDHa
L. lactis strain | Initial glucose (%) | Nisin (ng/ml) | Induction (OD600) | Ox | End products
|
Recovery | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Lactate | Formate | Acetate | Acetoin | 2,3-Butanediol | Ethanol | Mannitol | ||||||
NZ9000(pNZ8148) | 0.5 | 0 | 0.5 | − | 1.96 | 0.11 | 0.01 | <0.02 | <0.01 | 0.08 | <0.003 | 1.0 |
0.5 | 1 | 0.5 | − | 1.93 | 0.09 | 0.02 | <0.02 | <0.01 | 0.07 | <0.003 | 1.0 | |
NZ9000(pWW003) | 0.5 | 0 | 0.5 | − | 1.93 | 0.10 | 0.04 | <0.02 | <0.01 | 0.04 | <0.003 | 1.0 |
0.5 | 0.1 | 0.5 | − | 1.85 | 0.15 | 0.06 | <0.02 | <0.01 | 0.05 | 0.03 | 1.0 | |
0.5 | 0.3 | 0.5 | − | 1.79 | 0.16 | 0.08 | <0.02 | <0.01 | 0.06 | 0.05 | 1.0 | |
0.5 | 1 | 0.5 | − | 1.66 | 0.14 | 0.08 | <0.02 | <0.01 | 0.03 | 0.10 | 0.98 | |
0.5 | 3 | 0.5 | − | 1.65 | 0.12 | 0.09 | <0.02 | <0.01 | 0.02 | 0.10 | 0.98 | |
0.5 | 10 | 0.5 | − | 1.63 | 0.10 | 0.08 | <0.02 | <0.01 | 0.02 | 0.11 | 0.98 | |
0.5 | 3 | 0.5 | + | 1.39 | <0.02 | 0.27 | 0.02 | <0.01 | <0.02 | 0.08 | 0.94 | |
HWA217(pWW003) | 0.5 | 0 | 0.5 | − | 2.01 | 0.22 | 0.08 | <0.02 | <0.01 | 0.03 | 0.036 | 1.1 |
0.5 | 0.1 | 0.5 | − | 1.95 | 0.18 | 0.08 | <0.02 | <0.01 | <0.02 | 0.038 | 1.1 | |
0.5 | 0.3 | 0.5 | − | 1.92 | 0.19 | 0.08 | <0.02 | <0.01 | <0.02 | 0.043 | 1.1 | |
0.5 | 1 | 0.5 | − | 1.79 | 0.19 | 0.11 | <0.02 | <0.01 | <0.02 | 0.10 | 1.1 | |
0.5 | 3 | 0.5 | − | 1.55 | 0.16 | 0.15 | <0.02 | <0.01 | <0.02 | 0.20 | 1.0 | |
0.5 | 10 | 0.5 | − | 1.59 | 0.15 | 0.16 | <0.02 | <0.01 | <0.02 | 0.21 | 1.0 | |
0.5 | 3 | 0.5 | + | 1.33 | <0.02 | 0.53 | 0.02 | <0.01 | <0.02 | 0.03 | 0.97 | |
NZ9010(pWW003) | 0.5 | 0 | 0.5 | − | 0.28 | 1.14 | 0.40 | 0.04 | 0.22 | 0.78 | 0.01 | 1.0 |
0.5 | 0.1 | 0.5 | − | 0.20 | 1.03 | 0.42 | 0.04 | 0.18 | 0.67 | 0.09 | 0.96 | |
0.5 | 0.3 | 0.5 | − | 0.17 | 1.00 | 0.43 | 0.05 | 0.16 | 0.61 | 0.14 | 0.96 | |
0.5 | 1 | 0.5 | − | 0.16 | 0.73 | 0.31 | 0.11 | 0.11 | 0.45 | 0.26 | 0.94 | |
0.5 | 3 | 0.5 | − | 0.18 | 0.76 | 0.33 | 0.13 | 0.11 | 0.48 | 0.27 | 1.0 | |
0.5 | 10 | 0.5 | − | 0.19 | 0.79 | 0.34 | 0.12 | 0.10 | 0.47 | 0.25 | 0.98 | |
0.5 | 3 | 0.5 | + | 0.02 | <0.02 | 0.41 | 0.57 | <0.01 | <0.02 | 0.10 | 0.88 | |
0.5 | 3 | 0.1 | − | 0.12 | 0.71 | 0.46 | 0.04 | 0.07 | 0.31 | 0.39 | 0.94 | |
2.0b | 0 | 0.1 | − | 0.95 | 0.59 | 0.07 | 0.10 | 0.19 | 0.48 | 0.03 | 1.1 | |
2.0b | 3 | 0.1 | − | 0.20 | 0.32 | 0.28 | 0.04 | 0.16 | 0.15 | 0.50 | 1.0 |
The initial glucose concentration, amount of nisin used for induction (in nanograms per milliliter), induction OD600 values, culture conditions (oxic or anoxic), and the product concentrations in moles produced per mole of glucose are presented. Ox, oxic conditions.
Glucose was not completely consumed (see Fig. 3).