Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 4;141(7):766–786. doi: 10.1182/blood.2022017046

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Figure 4. — C1Q⁺populations are cancer-initiating cells. (A-B) Pictures of PDX recipient-mice constructed by injecting 1 million primary cells of P-S1022. Red circles and arrows indicate the leukemia cutis. (C-D) The leukemic invasions in BM, spleen, liver, and cutis were analyzed by H&E staining (C-D), or IHC staining of hCD45 and hC1Q in liver (D). Flow cytometry analysis of human CD45, CD33, CD34, and C1Q (hCD45, hCD33, hCD34, and hC1Q) expression on cells recovered from BM (E), cutis (F), or spleen (G) of PDX recipient mice. (H) Flow cytometry sorting of BM and peripheral blood sample of P-S1022 based on expression of C1Q. (I-J) Survival curve and body weight of PDX mice injected with one million cells sorted from P-S1022. The hCD45⁺ cells of P-S1022 were sorted to deplete C1Q⁺ cells and injected into NSG mice through tail vein. P value was calculated by log-rank (Mantel-Cox) test (I) and 2-sample t test (J). (K) The leukemia cutis from PDX mice was analyzed by H&E and IHC staining of hCD45. Flow cytometry analysis of C1Q expression on cells is shown (right).

Figure 4. — C1Q⁺populations are cancer-initiating cells. (A-B) Pictures of PDX recipient-mice constructed by injecting 1 million primary cells of P-S1022. Red circles and arrows indicate the leukemia cutis. (C-D) The leukemic invasions in BM, spleen, liver, and cutis were analyzed by H&E staining (C-D), or IHC staining of hCD45 and hC1Q in liver (D). Flow cytometry analysis of human CD45, CD33, CD34, and C1Q (hCD45, hCD33, hCD34, and hC1Q) expression on cells recovered from BM (E), cutis (F), or spleen (G) of PDX recipient mice. (H) Flow cytometry sorting of BM and peripheral blood sample of P-S1022 based on expression of C1Q. (I-J) Survival curve and body weight of PDX mice injected with one million cells sorted from P-S1022. The hCD45⁺ cells of P-S1022 were sorted to deplete C1Q⁺ cells and injected into NSG mice through tail vein. P value was calculated by log-rank (Mantel-Cox) test (I) and 2-sample t test (J). (K) The leukemia cutis from PDX mice was analyzed by H&E and IHC staining of hCD45. Flow cytometry analysis of C1Q expression on cells is shown (right).