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Production of Man5K by C. acetobutylicum. Western blot analysis of SDS-PAGE with antiserum raised against Man5K (anti-K) (A) or biotin-labeled miniCipC1 (b-mC1) (B) K, whole form of Man5K produced in E. coli (0.1 mg/ml); tK, truncated form of Man5K produced in E. coli (0.1 mg/ml); lane 1, concentrated supernatant of C. acetobutylicum(p954-cipC1-K); lane 2, concentrated supernatant of C. acetobutylicum(p954-K); lane 3, concentrated supernatant of C. acetobutylicum (p952-cipC1).
