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. 2005 Mar;71(3):1215–1222. doi: 10.1128/AEM.71.3.1215-1222.2005

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FIG. 4. — Cellulose binding assay. The proteins adsorbed on Avicel were analyzed by Western blotting with antisera raised against the CBM3A of miniCipC1 (A) or against Man5K (B). (A) mC1, purified miniCipC1 (produced in E. coli); lane 1, cellulose-adsorbed fraction of C. acetobutylicum(p952-cipC1) supernatant; lane 2, cellulose-adsorbed fraction of C. acetobutylicum(p954-K) supernatant; lane 3, cellulose-adsorbed fraction of C. acetobutylicum(p954-cipC1-K) supernatant. (B) K, whole Man5K purified from E. coli; tK, purified truncated Man5K produced by E. coli; lane 1, cellulose-adsorbed fraction of C. acetobutylicum(p952-cipC1) supernatant; lane 2, cellulose-adsorbed fraction of C. acetobutylicum(p954-K) supernatant; lane 3, cellulose-adsorbed fraction of C. acetobutylicum(p954-cipC1-K) supernatant.