TABLE 1.
Cell number and percentage of the total cell count for HNA and LNA subpopulations in four salt marsh bacterioplankton samples after 24 h of incubationa
| Sample | Population | Control (avg ± SD)
|
DMSP (avg ± SD)
|
Significant differencec | ||||
|---|---|---|---|---|---|---|---|---|
| No. of cells (106 ml−1) | % of total cells | No. of cells sorted (103)b | No. of cells (106 ml−1) | % of total cells | No. of cells sorted (103)b | |||
| May-02 | HNA | 0.1 ± 0.1 | 2.1 ± 0.5 | 0.8 ± 0.1 | 5.2 ± 0.6 | 28.2 ± 1.7 | 49.3 ± 1.8 | Yes |
| LNA3 | 1.1 ± 0.3 | 16.8 ± 1.0 | 9.2 ± 0.3 | 6.4 ± 0.4 | 34.6 ± 1.0 | 59.2 ± 1.5 | Yes | |
| LNA2 | 5.0 ± 0.2 | 76.8 ± 0.8 | 27.2 ± 1.7 | 5.4 ± 0.4 | 29.2 ± 0.8 | 24.8 ± 0.9 | Yes | |
| LNA1 | 0.3 ± 0.1 | 4.4 ± 0.5 | 1.6 ± 0.1 | 1.5 ± 0.5 | 8.0 ± 1.8 | 6.6 ± 0.3 | No | |
| Sept-02 | HNA | 0.1 ± 0.1 | 2.1 ± 0.5 | 11.8 ± 1.9 | 5.8 ± 1.5 | 9.7 ± 2.6 | 80.2 ± 25.0 | Yes |
| LNA2 | 0.2 ± 0.1 | 11.2 ± 1.0 | 62.9 ± 0.6 | 40.7 ± 1.5 | 68.6 ± 2.5 | 566.2 ± 79.8 | Yes | |
| LNA1 | 1.5 ± 0.1 | 80.5 ± 1.6 | 455.9 ± 50.0 | 9.1 ± 0.3 | 15.4 ± 0.5 | 126.4 ± 11.6 | Yes | |
| Jan-03 | LNA2 | 2.6 ± 0.2 | 59.2 ± 1.9 | 78.1 ± 2.5 | 3.4 ± 0.2 | 56.1 ± 2.7 | 96.0 ± 5.2 | No |
| LNA1 | 1.8 ± 0.3 | 40.8 ± 5.1 | 53.9 ± 7.1 | 2.7 ± 0.2 | 43.9 ± 3.5 | 75.1 ± 9.8 | No | |
| May-03 | LNA2 | 5.5 ± 0.8 | 64.6 ± 3.9 | 99.5 ± 7.1 | 11.4 ± 0.9 | 63.6 ± 5.0 | 82.0 ± 8.2 | No |
| LNA1 | 3.2 ± 0.2 | 36.7 ± 4.4 | 56.5 ± 5.3 | 6.5 ± 1.0 | 36.1 ± 4.7 | 46.6 ± 7.6 | No | |
a
Averages and standard deviations were generated based on independent analysis of three replicate incubations.
b
Actual number of cells available for molecular analysis. Boldface indicates FCM populations that were able to provide DNA templates for subsequent molecular analysis.
c
“Significant difference” indicates whether the number of cells differed between DMSP and control treatments for a given subpopulation at P < 0.05.