TABLE 1.
Sample | Population | Control (avg ± SD)
|
DMSP (avg ± SD)
|
Significant differencec | ||||
---|---|---|---|---|---|---|---|---|
No. of cells (106 ml−1) | % of total cells | No. of cells sorted (103)b | No. of cells (106 ml−1) | % of total cells | No. of cells sorted (103)b | |||
May-02 | HNA | 0.1 ± 0.1 | 2.1 ± 0.5 | 0.8 ± 0.1 | 5.2 ± 0.6 | 28.2 ± 1.7 | 49.3 ± 1.8 | Yes |
LNA3 | 1.1 ± 0.3 | 16.8 ± 1.0 | 9.2 ± 0.3 | 6.4 ± 0.4 | 34.6 ± 1.0 | 59.2 ± 1.5 | Yes | |
LNA2 | 5.0 ± 0.2 | 76.8 ± 0.8 | 27.2 ± 1.7 | 5.4 ± 0.4 | 29.2 ± 0.8 | 24.8 ± 0.9 | Yes | |
LNA1 | 0.3 ± 0.1 | 4.4 ± 0.5 | 1.6 ± 0.1 | 1.5 ± 0.5 | 8.0 ± 1.8 | 6.6 ± 0.3 | No | |
Sept-02 | HNA | 0.1 ± 0.1 | 2.1 ± 0.5 | 11.8 ± 1.9 | 5.8 ± 1.5 | 9.7 ± 2.6 | 80.2 ± 25.0 | Yes |
LNA2 | 0.2 ± 0.1 | 11.2 ± 1.0 | 62.9 ± 0.6 | 40.7 ± 1.5 | 68.6 ± 2.5 | 566.2 ± 79.8 | Yes | |
LNA1 | 1.5 ± 0.1 | 80.5 ± 1.6 | 455.9 ± 50.0 | 9.1 ± 0.3 | 15.4 ± 0.5 | 126.4 ± 11.6 | Yes | |
Jan-03 | LNA2 | 2.6 ± 0.2 | 59.2 ± 1.9 | 78.1 ± 2.5 | 3.4 ± 0.2 | 56.1 ± 2.7 | 96.0 ± 5.2 | No |
LNA1 | 1.8 ± 0.3 | 40.8 ± 5.1 | 53.9 ± 7.1 | 2.7 ± 0.2 | 43.9 ± 3.5 | 75.1 ± 9.8 | No | |
May-03 | LNA2 | 5.5 ± 0.8 | 64.6 ± 3.9 | 99.5 ± 7.1 | 11.4 ± 0.9 | 63.6 ± 5.0 | 82.0 ± 8.2 | No |
LNA1 | 3.2 ± 0.2 | 36.7 ± 4.4 | 56.5 ± 5.3 | 6.5 ± 1.0 | 36.1 ± 4.7 | 46.6 ± 7.6 | No |
Averages and standard deviations were generated based on independent analysis of three replicate incubations.
Actual number of cells available for molecular analysis. Boldface indicates FCM populations that were able to provide DNA templates for subsequent molecular analysis.
“Significant difference” indicates whether the number of cells differed between DMSP and control treatments for a given subpopulation at P < 0.05.