TABLE 3.
Experimental design of the study—sample collection
| Day | Trial(s) | Sample type (no. of samples) and time of collectiona |
|---|---|---|
| 1 | 1 and 2 | Shavings (4), feed (1), water (1); before poults were placed in pens |
| 1 | 1 | Cloacal swabsb (12 or 13; 1/poult); when poults were placed in pens and before they were dosed with serovar Newport (pens 1 to 3) and E. coli (pen 3) |
| 2 | 2 | Cloacal swabs (12 or 13; 1/poult); after poults were dosed with serovar Newport (pens 1 to 3) and E. coli (pen 3) on day 1 but before CTF (pen 1) or saline (pen 2) treatment on day 2 |
| 4 | 1 and 2 | Litter (4), feed with litter (1), water (1), cloacal swabs (12 or 13; 1/poult) |
| 10 | 1 and 2 | Litter (4), feed with litter (1), water (1), cecal contentsc (12 or 13; 1/poult) |
a
Samples were taken from 12 or 13 poults in each of four pens.
b
Cloacal swabs were taken by inserting a cotton swab wetted with BPW into the cloaca.
c
Upon necropsy; cecal contents were taken and examined for the presence of Salmonella and E. coli; one isolate each of E. coli and serovar Newport per sample, if present, was further characterized.