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. 2023 Nov 15;14(11):745. doi: 10.1038/s41419-023-06269-y

Fig. 8. NR2F1 bound to the promoter region of MMP2, MMP9, and VE-cadherin.

Fig. 8

A Relative mRNA expression of MMP2, MMP9, and VE-cadherin was tested in U87 and U251 cells with overexpressing or knockdown NR2F1 by qPCR assay (n = 3). *P < 0.05 vs OV-NC group; #P < 0.05 vs sh-NC group. B Relative expression of VM-related protein was shown (n = 3). *P < 0.05, **P < 0.01 vs OV-NC group; #P < 0.05 vs sh-NC group. C–E Schematic representation of the MMP2, MMP9, and VE-cadherin promoter region 1000 bp upstream of TSS, which was designated as +1, was shown. The putative NR2F1 binding site was indicated. PCR was conducted with the resulting precipitated DNA. F–H Schematic depiction of the different reporter plasmids and relative luciferase activity. The relative luciferase activity was performed after cells were co-transfected with the promoter (−1000 to 0 bp) (or promoter-deleted putative NR2F1 binding site) with pGL3 empty promoter or pGL3-NR2F1 (n = 3). *P < 0.05, **P < 0.01 vs pGL3-NR2F1 group.