(A) RLB based on the 16S rRNA of different species of the genus Borrelia and R57. Specific probes for the genus Borrelia and R57 were used in this assay. Lanes: 1, B. japonica; 2, B. burgdorferi sensu stricto; 3, B. garinii; 4, B. afzelii; 5, B. valaisiana; 6, B. lusitaniae; 7, Spanish relapsing fever spirochete; 8, B. turicatae; 9, B. duttonii; 10, Borrelia anserina; 11, B. hermsii; 12, B. crocidurae; 13, B. recurrentis; 14, B. hispanica; 15, R57. (B) Hybridization with samples from small mammal tissues. The upper spots in each panel correspond to the 16S genetic probe for Borrelia spp.; the lower spots correspond to the 16S-specific probe for R57 spirochetes.