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. 2023 Nov 15;13:19927. doi: 10.1038/s41598-023-43099-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Cap on BO-induced phospho-PLB and phospho-RyR2 in the heart of BO mice. (A) Expression of phospho-PLB (Thr-17) was significantly increased in the BO group (n = 4), but this increase was blocked in the BO + Cap group (n = 4). *P < 0.05 by one-way ANOVA followed by the Tukey–Kramer post hoc test. Images of full-size immunoblots are shown in Supplementary Fig. 17. (B) Expression of phospho-PLB (Ser-16) was significantly increased in the BO group (n = 5), but this increase was significantly blocked in the BO + Cap group (n = 5). *P < 0.05 by one-way ANOVA followed by the Tukey–Kramer post hoc test. Images of full-size immunoblots are shown in Supplementary Fig. 18. (C) Expression of phospho-RyR2 (Ser-2814) was significantly increased in the BO group (n = 5), but this increase was blocked in the BO + Cap group (n = 5). **P < 0.01 by one-way ANOVA followed by the Tukey–Kramer post hoc test. Data are presented as mean ± SD and dots show individual data. Images of full-size immunoblots are presented in Supplementary Fig. 19. (D) Expression of phospho-RyR2 (Ser-2808) was significantly increased in the BO group (n = 6), but this increase was blocked in the BO + Cap group (n = 6). **P < 0.01 by one-way ANOVA followed by the Tukey–Kramer post hoc test. Data are presented as mean ± SD and dots show individual data. Images of full-size immunoblots are shown in Supplementary Fig. 20.