Table 2.
List of the top 25 most robustly regulated candidate proteins in WT-response vs ∆tmk1-response (at least one isoform has a |Log2FC| > 2).
| No. | Protein ID | Log2FC | phosphorylation | Annotation | FunCat number (second rank) | FunCat description |
|---|---|---|---|---|---|---|
| 1 | 154783 | − 11.42 | Nitrilase |
01.02// 32.05//32.07// 36.20 |
METABOLISM//nitrogen, sulfur and selenium metabolism// CELL RESCUE, DEFENSE AND VIRULENCE//disease, virulence and defense//detoxification// SYSTEMIC INTERACTION WITH THE ENVIRONMENT//plant / fungal specific systemic sensing and response |
|
| − 4.57 | ||||||
| − 1.79* | ||||||
| 2 | 162609 | 3.87 | Putative actin binding protein |
14.10// 16.01//16.17// 18.02// 20.09// 30.01//30.05//40.01//42.04// 43.01 |
PROTEIN FATE (folding, modification, destination)//assembly of protein complexes// PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT//protein binding//metal binding// REGULATION OF METABOLISM AND PROTEIN FUNCTION//regulation of protein activity// CELLULAR TRANSPORT, TRANSPORT FACILITIES AND TRANSPORT ROUTES//transport routes// CELLULAR COMMUNICATION/SIGNAL TRANSDUCTION MECHANISM//cellular signaling//transmembrane signal transduction//CELL FATE//cell growth / morphogenesis// BIOGENESIS OF CELLULAR COMPONENTS//cytoskeleton/structural proteins//CELL TYPE DIFFERENTIATION//fungal/microorganismic cell type differentiation |
|
| 3 | 210492 | 3.76 | x | Protein with tetratricopeptide repeat-containing domain |
10.03//12.04// 42.16 |
CELL CYCLE AND DNA PROCESSING//cell cycle//PROTEIN SYNTHESIS//translation// BIOGENESIS OF CELLULAR COMPONENTS//mitochondrion |
| 4 | 298020 | − 3.59 | Microtubule-binding protein | 12.01//16.01//40.10// | PROTEIN SYNTHESIS//ribosome biogenesis//PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT (structural or catalytic)//protein binding//CELL FATE//cell death | |
| 1.06 | ||||||
| 1.22 | ||||||
| 5 | 131489 | − 3.41 | Putative epoxide hydrolase | 01.05//32.07//32.10 | METABOLISM//C-compound and carbohydrate metabolism//CELL RESCUE, DEFENSE AND VIRULENCE//detoxification//degradation / modification of foreign (exogenous) compounds | |
| 6 | 300623 | − 3.00 | Candidate isovaleryl-CoA dehydrogenase | 01.01//01.06//02.11//02.25 | METABOLISM//amino acid metabolism//lipid, fatty acid and isoprenoid metabolism//ENERGY//electron transport and membrane-associated energy conservation//oxidation of fatty acids | |
| 7 | 298303 | 2.84 | Putative UDP-glucose pyrophosphorylase |
01.03//01.04//01.05//02.01//02.19// 14.07 |
METABOLISM//nucleotide/nucleoside/nucleobase metabolism//phosphate metabolism//C-compound and carbohydrate metabolism//ENERGY//glycolysis and gluconeogenesis//metabolism of energy reserves (e.g. glycogen, trehalose)//PROTEIN FATE (folding, modification, destination)//protein modification | |
| − 1.12 | ||||||
| 8 | 142072 | 2.78 | x | Protein with protein-kinase like domain | 99 | UNCLASSIFIED PROTEINS |
| 9 | 298717 | − 2.63 | Putative flavohemoprotein | 01.02//02.13//20.01//32.01//32.05 | METABOLISM//nitrogen, sulfur and selenium metabolism//ENERGY//respiration//CELLULAR TRANSPORT, TRANSPORT FACILITIES AND TRANSPORT ROUTES//transported compounds (substrates)//CELL RESCUE, DEFENSE AND VIRULENCE//stress response//disease, virulence and defense | |
| 10 | 299691 | − 2.61 | x | Formamidase-like protein | 01.02 | METABOLISM//nitrogen, sulfur and selenium metabolism |
| 11 | 302658 | − 2.39* | Putative NADP( +)-dependent dehydrogenase |
01.05//01.06// 16.21 |
METABOLISM//C-compound and carbohydrate metabolism//lipid, fatty acid and isoprenoid metabolism// PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT//complex cofactor/co-substrate/vitamin binding |
|
| 12 | 137374 | − 2.33 | Related to phospholipase A-2-activating protein |
01.06// 10.01//10.03// 11.02// 14.13// 16.01// 18.02 |
METABOLISM//lipid, fatty acid and isoprenoid metabolism// CELL CYCLE AND DNA PROCESSING//DNA processing//cell cycle// TRANSCRIPTION//RNA synthesis PROTEIN FATE (folding, modification, destination)//protein/peptide degradation// PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT//protein binding// REGULATION OF METABOLISM AND PROTEIN FUNCTION//regulation of protein activity |
|
| 13 | 302156 | − 2.32 | NAD dependent formate dehydrogenase | 01.01//01.05//01.07//02.16 |
METABOLISM//amino acid metabolism//C-compound and carbohydrate metabolism//metabolism of vitamins, cofactors, and prosthetic groups// ENERGY//fermentation |
|
| − 1.69 | ||||||
| − 1.63 | ||||||
| − 1.51 | ||||||
| 14 | 150014 | − 2.29 | x | Putative aconitate hydratase |
01.01//01.05// 02.10//02.16// 16.21// |
METABOLISM//amino acid metabolism//C-compound and carbohydrate metabolism// ENERGY//tricarboxylic-acid pathway (citrate cycle, Krebs cycle, TCA cycle)//fermentation// PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT//complex cofactor/cosubstrate/vitamine binding |
| − 1.55 | ||||||
| − 1.24 | ||||||
| 15 | 299540 | − 2.29 | x | Hypothetical protein | 01.01//01.20 | METABOLISM//amino acid metabolism//secondary metabolism |
| − 2.08 | ||||||
| − 1.80 | ||||||
| 16 | 297668 | 2.22 | Peroxisomal catalase |
16.21// 20.01// 32.01//32.07 |
PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT//complex cofactor/cosubstrate/vitamine binding//CELLULAR TRANSPORT, TRANSPORT FACILITIES AND TRANSPORT ROUTES//transported compounds (substrates)// CELL RESCUE, DEFENSE AND VIRULENCE//stress response//detoxification |
|
| 17 | 131489 | − 2.22 | Putative epoxide hydrolase |
01.05// 32.07//32.10 |
METABOLISM//C-compound and carbohydrate metabolism// CELL RESCUE, DEFENSE AND VIRULENCE//detoxification//degradation / modification of foreign (exogenous) compounds |
|
| 18 | 268974 | − 2.12 | Putative short-chain dehydrogenase/ reductase | 01.05//01.06//01.20//16.21 |
METABOLISM//C-compound and carbohydrate metabolism//lipid, fatty acid and isoprenoid metabolism//secondary metabolism// PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT//complex cofactor/cosubstrate/vitamine binding// |
|
| 1.51 | ||||||
| 1.39* | ||||||
| 1.27 | ||||||
| 19 | 302655 | − 2.10 | GMC oxidoreductase AA3_2 |
01.06//01.07// 11.04// 14.13// 16.01//16.03// 34.11// |
METABOLISM//lipid, fatty acid and isoprenoid metabolism//metabolism of vitamins, cofactors, and prosthetic groups//TRANSCRIPTION//RNA processing// PROTEIN FATE (folding, modification, destination)//protein/peptide degradation// PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT//protein binding//nucleic acid binding// INTERACTION WITH THE ENVIRONMENT//cellular sensing and response to external stimulus |
|
| 20 | 302177 | 2.06 | x | Branched-chain-amino-acid aminotransferase |
01.01// 16.21// |
METABOLISM//amino acid metabolism PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT (structural or catalytic)//complex cofactor/cosubstrate/vitamine binding |
| 21 | 150078 | 2.04 | Putative pyruvate decarboxylase |
01.01//01.05//01.07//02.13//02.16// 11.02 |
METABOLISM//amino acid metabolism//C-compound and carbohydrate metabolism//metabolism of vitamins, cofactors, and prosthetic groups// ENERGY//respiration//fermentation// TRANSCRIPTION//RNA synthesis |
|
| 22 | 297147 | 2.04 | Putative Rho GDP-dissociation inhibitor |
16.01//18.01//18.01.07//18.02//30.01// 34.07//40.01// 42.04// 43.01 |
PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT//protein binding//REGULATION OF METABOLISM AND PROTEIN FUNCTION//regulation by//CELLULAR COMMUNICATION/SIGNAL TRANSDUCTION MECHANISM//cellular signaling//INTERACTION WITH THE ENVIRONMENT//cell adhesion// CELL FATE//cell growth / morphogenesis// BIOGENESIS OF CELLULAR COMPONENTS//cytoskeleton/structural proteins// CELL TYPE DIFFERENTIATION//fungal/microorganismic cell type differentiation |
|
| 23 | 146044 | − 2.03* | Putative aminotransferase |
01.01// 16.21 |
METABOLISM//amino acid metabolism// PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT//complex cofactor/cosubstrate/vitamine binding |
|
| − 1.96 | ||||||
| − 1.09* | ||||||
| 24 | 297025 | − 2.03 | NADP-dependent glutamate dehydrogenase |
01.01//01.02// 02.10// 16.21// 42.01 |
METABOLISM//amino acid metabolism//nitrogen, sulfur and selenium metabolism// ENERGY//tricarboxylic-acid pathway (citrate cycle, Krebs cycle, TCA cycle)// PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT//complex cofactor/cosubstrate/vitamine binding//BIOGENESIS OF CELLULAR COMPONENTS//cell wall |
|
| − 1.60* | ||||||
| 25 | 52055 | − 1.96 | Putative isocitrate dehydrogenase |
01.01//01.05//01.06//01.07// 02.10//02.45// 16.21 |
METABOLISM//amino acid metabolism//C-compound and carbohydrate metabolism//lipid, fatty acid and isoprenoid metabolism//metabolism of vitamins, cofactors, and prosthetic groups//ENERGY//tricarboxylic-acid pathway (citrate cycle, Krebs cycle, TCA cycle)//energy conversion and regeneration// PROTEIN WITH BINDING FUNCTION OR COFACTOR REQUIREMENT//complex cofactor/cosubstrate/vitamine binding |
Asterisk indicates proteins tentatively identified (statistical significance of search results for MS and MS/MS spectra not given, comparison of individual MS/MS spectra strongly indicate correct identification), Log2FC changes for protein isoforms are listed with the respective protein ID.