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Effects of LIPUS treatment on iNOS and Arg‐1 expression. Microglia were treated with LPS, IL‐4, LIPUS, or a combination of LIPUS and LPS or IL‐4. Representative changes in (A) iNOS and (B) Arg‐1 mRNA were detected by qRT‐PCR at 4 h after treatment with LIPUS. The relative levels of (C) iNOS and (D) Arg‐1 protein were detected by western blotting at 4 h after treatment with LIPUS. *p < 0.05; **p < 0.01; ***p < 0.001; N.S. = not significant; n = 8.
