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LIPUS treatment regulates M1/M2 microglial polarization. (A) Representative images of immunofluorescent labeling of CD68 (red), CD206 (green), and DAPI (blue) staining of microglia were treated with LPS, IL‐4, LIPUS, or a combination of LIPUS and LPS or IL‐4. The levels of expression of M1 and M2 cell markers (B) CD68 and (C) CD206 were quantified using immunofluorescence at 4 h after treatment with LIPUS. Scale bar = 150 μm. *p < 0.05; **p < 0.01; N.S. = not significant; n = 6.
