TABLE 2.
Primary and secondary antibodies used in this study.
| Antibody | Lot # | Source | Dilution/Concentration |
|---|---|---|---|
| Mouse anti‐GCPII | GTX80151 | GeneTex | 1:1000 (WB), 1:200 (IF) |
| Rabbit anti‐GFAP | 80,788 | Cell Signal | 1:1000 (WB), 1:500 (IHC) |
| Chicken anti‐GFAP | ab4674 | Abcam | 1:200 (IF) |
| Rabbit anti‐IBA1 | ab178846 | Abcam | 1:1000 (WB), 1:200 (IF), 1:500 (IHC) |
| Rabbit anti‐Caspase3 | 9662 | Cell Signal | 1:1000 (WB) |
| Rabbit anti‐cleaved Caspase3 | 9664 | Cell Signal | 1:1000 (WB) |
| Rabbit anti‐Bax | 2774 | Cell Signal | 1:1000 (WB), 1:200 (IF) |
| Rabbit anti‐GAPDH | AF7021 | Affinity | 1:2000 (WB) |
| Rabbit anti‐beta Actin | AF7018 | Affinity | 1:2000 (WB) |
| Goat Anti‐Rabbit IgG HRP | S0001 | Affinity | 1:5000 (WB) |
| Goat Anti‐Mouse IgG HRP | S0002 | Affinity | 1:5000 (WB) |
| Goat Anti‐Mouse AlexaFluor 488 | 115–545‐003 | Jackson | 1:200 (IF) |
| Goat Anti‐Rabbit AlexaFluor 594 | 111–585‐003 | Jackson | 1:200 (IF) |
| Rabbit Anti‐Chicken AlexaFluor 647 | 303–605‐003 | Jackson | 1:200 (IF) |