Table 1.
Regression and validation data for the determination of harmaline and harmine using the proposed method.
| Parameters | Harmaline | Harmine |
|---|---|---|
| Wavelength (nm) | 419 | 456 |
| Linearity range (ng/mL) | 10–200 | 10–200 |
| LOD (ng/mL) | 2.51 | 1.82 |
| LOQ (ng/mL) | 7.53 | 5.46 |
| Slope | 0.011 | 0.024 |
| Intercept | 0.762 | 0.750 |
| Coefficient of determination (r2) | 0.9998 | 0.9997 |
| Accuracy (%R)a | 98.20 | 99.65 |
| Repeatability Precision (RSD)b | 0.805 | 0.878 |
| Intermediate precision (RSD)b | 0.780 | 0.799 |
| Robustness (%R ± RSD) | ||
| Buffer pH (± 0.5) | 100.33 ± 0.804 | 98.55 ± 0.651 |
| Buffer volume (± 0.25 mL) | 98.26 ± 0.708 | 99.20 ± 0.840 |
| HP-β-CD volume (± 0.1 mL) | 98.54 ± 0.698 | 100.24 ± 0.925 |
aAverage of nine determinations (three concentrations repeated three times).
bRSD of nine determinations (three concentrations repeated three times).