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. 2023 Nov 15;14:7384. doi: 10.1038/s41467-023-42390-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Representative confocal microscopy images of immunofluorescence (IF) assay in MRC5 cells showing, after 16 J/m² UV-C irradiation, the localization of RNAP1 (green) and FBL (red) in transformed fibroblasts, at different times Post UV-Irradiation (PUVI). Nuclei and nucleoli are indicated by dashed and dotted lines respectively. Scale bar: 5 µm. b Quantification of cell number for RNAP1 localization (inside the nucleolus, outside the nucleolus or mixed localization) at different times PUVI. At least 50 cells from one representative experiment were analyzed. c Western Blot of SMN and CSB on whole cell extracts of transformed fibroblasts (MRC5-SV cells). Doxycycline treatment induces the expression of the ShRNA against SMN. d Quantification of RNA-FISH assay showing the 47 S pre-rRNA level after UV-C exposure in transformed fibroblasts. Data are represented as mean values +/− SEM. At least 27 cells was quantified from one representative experiment. The p-value correspond to a student’s test with two-tailed distribution and two-sample unequal variance to compare after irradiation with No UV condition. Source data of uncropped gel and graphs are provided as a Source Data file.