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. 2023 Nov 15;14:7384. doi: 10.1038/s41467-023-42390-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, d Representative microscopy images of immunofluorescence (IF) assay showing the localization of SMN (red) and (a) RNAP1 or (d) FBL (green) at different times Post UV-Irradiation (PUVI) in cells transfected with siMock or siCoilin pool. Nuclei and nucleoli are indicated by dashed and dotted lines respectively. Scale bar: 5 µm. b, c Quantification of cells number for localization of (b) SMN (in Cajal Bodies [CBs] or Gems, at the periphery of the nucleolus or mixed localization) and (c) RNAP1 (inside the nucleolus, outside the nucleolus or mixed localization) at different times PUVI in cells transfected with siMock or siCoilin pool. At least 35 cells from one representative experiment were analyzed. e Representative microscopy images of Proximity Ligation Assay (PLA) in red showing the interaction between SMN and FBL at different times PUVI in cells transfected with siMock or siCoilin pool. Scale bar: 5 µm. DAPI in grey. f Quantification of fluorescent signal in the nucleus against the couple SMN-FBL from PLA experiment in cells transfected with siMock or siCoilin pool after UV-C irradiation. Data are represented as mean values +/− SEM. At least 45 cells was quantified from one representative experiment. The p-value correspond to a student’s test with two-tailed distribution and two-sample unequal variance to compare siCoilin with siMock. Source data of graphs are provided as a Source Data file.