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Effects of IL-18, IL-1, and TNF-α on p47phox phosphorylation. 32P-labeled neutrophils were incubated with IL-18 (500 ng/ml), TNF-α (100 U/ml), or IL-1β (50 ng/ml) for 45 min. p47phox was immunoprecipitated and analyzed by SDS-PAGE transfer and revealed by autoradiography ([32P]p47phox) with an anti-p47phox antibody. Data are representative of those from three experiments.
