FIG. 6.

Effects of colchicine on CD11b expression and the PMN oxidative burst after IL-18 priming. Samples were preincubated at 37°C with colchicine at various concentrations for 5 min before the addition of IL-18 (500 ng/ml) for 45 min. CD11b expression was measured with a mouse anti-human FITC-anti-CD11b antibody. The oxidative burst was measured by incubation of the samples with hydroethidine for 15 min before the addition of IL-18 and then stimulation with fMLP (10⁻⁶ M) for 5 min. The mean fluorescence intensity of the FITC-anti-CD11b antibody and the ethidium content were recorded as described in Materials and Methods. Values are expressed as means ± SEMs (n = 3). *, significantly different from the results for the samples without colchicine (P < 0.05).