TABLE 2.

Effects of BDM on PMN oxidative burst and fMLP binding after IL-18 activation

BDM concn (mM)	Mean fluorescence intensity of:
	E+ contenta		FITC-fMLPb
	PBS	IL-18 + fMLP	PBS	IL-18
0	15.8 ± 1.0	43.5 ± 5.6c	58.3 ± 1.9	43.7 ± 1.3c
0.1	13.0 ± 1.0	59.0 ± 1.0c,d	56.0 ± 1.5	40.0 ± 3.2c,d
1	15.0 ± 0.6	67.3 ± 6.2c,d	54.7 ± 1.7	33.0 ± 3.5c,d
5	15.5 ± 1.3	26.8 ± 3.5	55.7 ± 5.2	44.0 ± 5.7
10	15.5 ± 0.6	20.5 ± 3.1	55.0 ± 3.5	47.3 ± 4.9

^aSamples were preincubated at 37°C with BDM at various concentrations for 5 min before the addition of hydroethidine for 15 min; the samples were then exposed to IL-18 (500 ng/ml) for 45 min and stimulated with fMLP (10⁻⁶ M) for 5 min. The mean fluorescence intensity of E⁺ was recorded as described in Materials and Methods. Values are expressed as means ± SEMs (n = 3).

^bSamples were preincubated for 20 min at 37°C with BDM at various concentrations and were then exposed to IL-18 (500 ng/ml) for 45 min. The mean fluorescence intensity of FITC-fMLP was recorded as described in Materials and Methods. Values are expressed as means ± SEMs (n = 3).

^cSignificantly different from the results for the samples incubated with PBS instead of IL-18.

^dSignificantly different from the results for the samples incubated with PBS instead of BDM.