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. 2023 Oct 27;150(21):dev201593. doi: 10.1242/dev.201593

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 7. — Localization of DYNLL1 and DYNLL2 in Actl7b-deficient testis. (A) Graphical depiction of DYNLL1 and DYNLL2 immunolocalization during spermiogenesis based on literature (Wang et al., 2005). (B) Western blots on protein extracts from whole Actl7b^+/+, Actl7b^+/− and Actl7b^−/− testis. Anti-ACTL7B, anti-DYNLL2 and anti-DYNLL2 were used. α-Tubulin was used as loading control. (C) DYNLL1 staining in Actl7b^+/+, Actl7b^+/− and Actl7b^−/− elongating spermatids. DAPI was used as a counterstain. Scale bar: 20 µm. (D) DYNLL2 staining in Actl7b^+/+, Actl7b^+/− and Actl7b^−/− elongating spermatids. DAPI was used as a counterstain. Scale bar: 20 µm. (E) Immunocytochemical staining against DYNLL2 in wild-type and ACTL7B-eGFP-expressing HEK cells. Scale bars: 50 µm. (F) Immunocytochemical staining against DYNLL1 in wild-type and ACTL7B-eGFP-expressing HEK cells. Scale bars: 50 µm. Images shown in E and F are taken from the overviews displayed in Figs S11 and S12.