FIG. 3.

Direct action of anisodamine on T cells or monocytes prepared from human PBMCs. (A) Human peripheral blood T cells (5 × 10⁵ cells) were stimulated with anti-CD3 and anti-CD28 antibodies in the presence or the absence of anisodamine for 48 h. The culture supernatants were assayed for cytokine levels by ELISA. The concentrations of anisodamine added were as follows: open bars, none; shaded bars, 25 μg/ml; solid bars, 50 μg/ml. (B) Reconstruction experiments were performed with isolated human peripheral blood T cells and monocytes. The compositions of the cells were as follows: open bars, T cells plus monocytes; shaded bars, T cells plus monocytes pretreated with anisodamine; solid bars, T cells pretreated with anisodamine plus monocytes. The mixed cells were stimulated with TSST-1 (10 ng/ml) for 48 h, and the culture supernatants were assayed for cytokines by ELISA.