FIG. 8.

Effects of anisodamine on proinflammatory and anti-inflammatory cytokine levels in serum after TSST-1 injection in mice. Groups of 12 mice were injected i.p. with 20 mg of d-galactosamine 1 h before an i.p. challenge with 10 μg of TSST-1. Treatment (i.p. injection) with anisodamine at 50 mg/kg of body weight was carried out 10 min after the TSST-1 injection. Sera were obtained periodically by cardiac tapping, and the cytokine levels in sera were determined by ELISA. (A) TNF-α; (B) IL-2; (C) IFN-γ; (D) IL-10. Anisodamine treatment of mice injected with TSST-1 was as follows: open circles, none; closed circles, 50 mg/kg. Open squares, no TSST-1 injection (control). *, P < 0.05.