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Reactivities of mouse MAbs with affinity-purified llama IgGs in ELISA. (A) IgG1; (B) IgG3; (C) IgG2. Twofold dilutions of supernatants were tested in triplicate wells against purified IgGs. Bound MAbs were detected with HRP-goat anti-mouse IgG. Columns represent mean optical densities for three replicate wells ± standard deviations of the means (I bars). Asterisks denote MAbs selected for further evaluation.
