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Specificities of MAbs for native camelid IgGs. The anti-llama IgG1, IgG3, and IgG2 MAbs, 27E10, 8E1, and 19D8, respectively, were coupled with CN-Br activated Sepharose 4B and used to purify IgGs from camelid sera. (A) Llama serum; (B) alpaca serum; (C) guanaco serum; (D) camel serum. Eluted IgG fractions were resolved by SDS-PAGE under nonreducing and reducing conditions and stained with Coomassie blue. Estimated molecular masses (in kilodaltons) are indicated.
