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Reactivities of HCAb-specific MAbs with eluates from the anti-IgG3 and anti-IgG2 affinity columns in Western blots. Affinity-purified HCAbs from llama (A) and alpaca (B) sera were resolved by SDS-PAGE, blotted onto nitrocellulose, and developed with anti-IgG2 and anti-IgG3 MAbs (20 μg/ml) or normal mouse serum. α-3, anti-IgG3 (8E1) MAbs; α-2, anti-IgG2 (19D8) MAbs; -ve, negative (normal mouse serum). Estimated molecular masses (in kilodaltons) are indicated.
