FIG. 2.
Determination of the lower limit of the RNA template concentration and optimization of MgCl2 concentrations for DD reactions. RNA from toluene-induced P. putida F1 cells was processed for DD. (A) Different amounts of template RNA. Lane 1, 100-bp ladder; lane 2, 15 ng; lane 3, 1.5 ng; lane 4, 0.15 ng; lane 5, 0.015 ng. (B) Effects of different MgCl2 concentrations on the complexity of the band pattern. Lane 1, 100-bp ladder; lane 2, 8 mM; lane 3, 4 mM; lane 4, 2 mM; lane 5, 1 mM. The position of the todC1 fragment is indicated by arrows.