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. 1998 Oct;64(10):3698–3706. doi: 10.1128/aem.64.10.3698-3706.1998

FIG. 3.

FIG. 3

Optimization of nucleotide and primer concentrations for DD reactions. RNA from toluene-induced P. putida F1 cells was processed for DD. (A) Effects of different nucleotide concentrations on the complexity of the band pattern. Lane 1, 100-bp ladder; lane 2, 200 μM; lane 3, 20 μM, lane 4, 2 μM; lane 5, 0.2 μM. (B) Effects of different primer concentrations on the complexity of the band pattern. Lane 1, 100-bp ladder; lane 2, 2 μM; lane 3, 0.2 μM; lane 4, 0.02 μM; lane 5, 0.002 μM. The position of the todC1 fragment is indicated by arrows.