FIG. 7.

DD analysis of toluene-induced P. putida F1 cells performed with arbitrary primers. RNAs from uninduced cells (lanes 2, 4, 7, and 9) or toluene-induced cells (lanes 3, 5, 8, and 10) were reverse transcribed by using primer 70.3. Subsequently, primer 70.3 alone (lanes 7 and 8), primer SD14 alone (lanes 9 and 10), or both primers (lanes 2 through 5) were used for PCR. Lanes 1 and 6 contained a 100-bp ladder. The position of the todC1C2 fragment in lanes 3 and 5 is indicated by an arrow.