FIG. 4.

Subpopulation behavior during LB medium starvation. Tetracycline-resistant (MG1655 zhj-3076::Tn10 or zah-281::Tn10) and kanamycin-resistant (MG1655 rha::Tn5 or purD81::Tn5) cells were separated by ultracentrifugation in the radioselectan solution. After separation, the tetracycline-resistant culture was divided into HD^T and LD^T subpopulations, and the kanamycin-resistant culture was divided into HD^K and LD^K subpopulations. (A) Evolution of MG1655 culturability during the stationary phase without (▴) or with (▵) the ultracentrifugation step. The open and solid symbols overlap. (B) Evolution of culturability of HD^T or HD^K cells alone (▪) or LD^T or LD^K cells alone incubated in LB_10H. (C) Evolution of culturability of a mixed population (25% LD^T cells and 75% HD^K cells) in LB_10H (final cell concentration, 5 × 10⁹ bacteria/ml). The experiments were repeated three times.