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. 2005 Apr;187(7):2233–2243. doi: 10.1128/JB.187.7.2233-2243.2005

FIG. 5.

FIG. 5.

Three-hybrid experiments. (a) Schematic maps of pUT18C-ftsB and pUT18C-ftsB/ftsL plasmids show the locations of the T18 gene-ftsB fusion and the ftsL and bla (ampicillin resistance) genes, the ColE1 origin of replication (ori ColE1), the Shine-Dalgarno sequence (SD), and the lac promoter (Pr lac). DHM1 cells were cotransformed with the pKT25 derivative expressing the indicated T25-Fts protein fusion, either plasmid pUT18C-ftsB, expressing the T18-FtsB fusion, or plasmid pUT18C-ftsB/ftsL, coexpressing the T18-FtsB fusion and the wild-type FtsL polypeptide. beta-Gal, β-galactosidase. (b) Schematic maps of pUT18C-ftsI and pUT18C-ftsI/ftsL plasmids show locations of the T18 gene-ftsI fusion and the ftsL and bla (ampicillin resistance) genes, the ColE1 origin of replication, the Shine-Dalgarno sequence, and the lac promoter. DHM1 cells were cotransformed with the indicated pKT25 plasmid, either pUT18C-ftsI, expressing the T18-FtsI fusion, or pUT18C-ftsB/ftsL, coexpressing the T18-FtsI fusion and the wild-type FtsL polypeptide. Efficiencies of the interactions were monitored as described in the legend to Fig. 3. Each bar represents the mean value from results for at least three independent cultures, with standard deviations below 20% of the mean.