Fig. 6.

Effects of endurance training on myokine and bone phenotype. (A) Schematic showing the setup of the muscle‐bone construct co‐cultures. (B) The 48‐hour endurance (END) exercise regimen consists of alternating 6‐hour stretch (1% strain) at 1/30 Hz and 6‐hour rest. (C) Heat maps showing the gene expression of muscle constructs subjected to END regimen when co‐cultured with bone constructs. (D) Gene expression heat maps of the corresponding bone constructs in the co‐cultures. All gene expressions were normalized to monoculture controls and housekeeping genes Gapdh (bone) and Ppia (muscle). n = 3 for stretch groups and n = 4 for static groups.