TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli DH5α | F−endA1 hsdR17(rk− mk+) thi-1 λ−recA1 gyrA96 relA1 Φ80dlacZΔM15 | Bethesda Research Laboratories, Rockville, Md. |
| P. multocida strains | ||
| X-73 | Serotype A:1 wild-type strain | 18 |
| AL362 | X-73 nrfE::tet(M) mutant | This study |
| AL464 | E. coli DH5α harboring pAL263 | This study |
| AL465 | AL362 harboring pAL99 | This study |
| AL466 | AL362 harboring pAL263 | This study |
| Plasmids | ||
| pAL99 | 40-bp EcoRI fragment containing P. multocida tpiA promoter region cloned into pPBA1100 EcoRI site | 17 |
| pAL209 | 2.1-kb insert containing nrfE cloned into pWSK129, Kanr | This study |
| pAL211 | pWSK129 containing the nrfE::tet(M) cassette, Kanr Tetr | This study |
| pAL263 | 2-kb insert containing full nrfE gene in pAL99 (nrfE expression driven by tpiA promoter) | This study |
| pBA1100 | P. multocida-E. coli shuttle vector, pUC18 derivative | 19 |
| pVB101 | tet(M) gene from Tn916 cloned into pBR322, Tetr Ampr | Vickers Burdett, Duke University, Durham, N.C. |
| pWSK129 | Low-copy-number E. coli vector, Kanr | 29 |