TABLE 2.
Oligonucleotides used in this study
| Oligonu- cleotide | Sequence (5′-3′)a | Positionb |
|---|---|---|
| 683 | CAATTTCAATCAATGCTCCCAC | NA |
| 1914 | GCACATGTCGACTGGATTGGGGTAG | 23284-23308 |
| 1915 | GGTAAATCTAGACATAAGCTTGGTTTT | 25218-25190 |
| 2106 | GCCCTTTCCGATAAATTGCAA | 1670646-1670626 |
| 2107 | ATCGCGGCTAATGGTGCTT | 1670549-1670567 |
| 2123 | AATGGCATGTTGGTCTTGTAAAAG-3′ | 20017-20040 |
| 2124 | CCACCACTTGCCCATGTTG | 20112-20094 |
| 2277 | GCCGGTAGTGTTGATGGA | 23172-23189 |
| 2278 | AGCAAGGCGCCTAGTGCC | 25411-25394 |
| 2283 | TTGATAGAGCTCTTCAAATTT | 23510-23530 |
| 2284 | CATACTCATGAGCTCACCTGT | 25494-25474 |
| 2363 | CCATCGCCGTTTATTTGTTCTT | 22553-22574 |
| 2364 | TGCCGTACAACGTATCACATGA | 22677-22656 |
| 2398 | GATTTTTGGCTTTACTGATCGCTT | 23560-23583 |
| 2399 | CGCAGTACCAATCATATACGGC | 23660-23639 |
| 2403 | GGACAAATGACGCTAGCATAAATG | 23449-23472 |
| 2404 | ACATCGCAAAGGCTTTATGCTAC | 23367-23389 |
| 2405 | AGCGTCATTTGTCCTGCATAGA | 23462-23441 |
| 2407 | AAGCCAAAAAACCAAGTTCTGG | 23571-23550 |
| 2442 | CACTATGATCCCAGAACTTGGTTTT | 23540-23564 |
a
Underlined bases indicate engineered restriction sites.
b
Position in the Pm70 genome (accession number NC_002663). NA, not applicable.