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TcpC and TcpQ localization patterns in tcpQ and tcpC deletion strains. (A) Immunoblot analysis of membrane and periplasmic fractions from wild-type (O395), ΔtcpQ (pNB10), and O395 (pNB10) strains with anti-TcpC antibody. (B) Fractionation profile of TcpQ in ΔtcpC (pNB11) and O395 (pNB11) strains with anti-TcpQ antibody. The first two lanes in both panels are wild-type and deletion strain controls. Per or per, periplasm; Sph, spheroplast; OM, outer membrane; IM, inner membrane.
