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. 2005 Apr;187(7):2225–2232. doi: 10.1128/JB.187.7.2225-2232.2005

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FIG. 5. — TcpC and TcpQ interact directly as demonstrated by metal affinity chromatography. (A) Metal affinity chromatography performed with His-tagged TcpC. Fractions were separated by SDS-PAGE, and TcpQ was detected by immunoblot analysis. Elution fractions 1 and 2 (E1 and E2) were obtained using buffer containing 150 mM imidazole. Fractions 3 and 4 (E3 and E4) were obtained using buffer containing 100 mM EDTA in order to completely strip the beads. (B) The reciprocal experiment with metal affinity chromatography with His-tagged TcpQ and probing of the immunoblot with anti-TcpC antibody. FT, flowthrough.