FIG. 6.

TcpC forms a high-molecular-weight complex similar to that seen for other secretins. The whole-cell lysates from wild-type (WT), ΔtcpC, ΔtcpQ, ΔtcpA, and ΔtcpS strains were resuspended in SDS-PAGE loading buffer and treated at 37 or 65°C or boiled for 5 min. Following SDS-PAGE, Western blot analysis with TcpC antibody was performed.