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. 2023 Nov 4;4(4):102691. doi: 10.1016/j.xpro.2023.102691

Table 1.

Nano-LC gradient profile

Time Flow (nL/min) Percent of B Percent of A
0 300 4 96
5 300 4 96
95 300 35 65
100 300 90 10
102.9 300 90 10
103 300 4 96
120 300 4 96

A: 99.9% H2O, 0.1% formic acid; B: 97.9% ACN, 2% H2O, 0.1% formic acid.