Table 2.
Orbitrap Lumos method summary
| Global settings | |
| Method duration (min) | 120 |
| Ion source type | NSI |
| Positive Ion (V) | 2200 |
| Ion transfer tube temp (oC) | 275 |
| Default charge state | 2 |
| Master scan | |
| Detector type | Orbitrap |
| Resolution | 120,000 |
| Scan range (m/z) | 350-1700 |
| Max injection time (ms) | 50 |
| AGC target | Standard |
| Data type | Profile |
| Filters | |
| Monoisotopic peak detection | Peptide |
| Intensity threshold | 1.0 × 104 |
| Include charge state | 2-6 |
| Exclude after n times | 1 |
| Exclusion duration (s) | 60 |
| Mass tolerance (ppm) | 10 |
| ddMS2 | |
| Data dependent mode | Cycle time |
| Time between master scans (s) | 2 |
| Isolation mode | Quadrupole |
| Isolation window (m/z) | 1.6 |
| Activation type | CID |
| Collision energy (%) | 30 |
| Detector type | Ion trap |
| Ion trap scan rate | Rapid |
| Max injection time (ms) | Dynamic |
| AGC target | Standard |