| Alkylation solution (110 mM iodoacetamide) | ||
|---|---|---|
| Reagent | Final concentration | Amount |
| Iodoacetamide | 110 mM | 200 mg |
| 25 mM NH4HCO3 | 25 mM | 10 mL |
| Total | N/A | 10 mL |
Prepare freshly, do not keep.
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