FIG. 3.

(A) Primer extension analysis of the nfrA transcript. Total RNA was isolated from ethanol-induced, mid-log-phase cells of S. aureus 8325. The product of the primer extension reaction was separated by electrophoresis under denaturing conditions alongside sequencing reaction mixtures with the same primer. The position of the transcription start site is indicated by an arrow. The transcript starts at an adenine. (B) Nucleotide sequence of the nfrA promoter. The sequence is compared to the B. subtilis σ^A promoter consensus sequence. Putative promoter elements are indicated by large boldface letters. Nucleotides deviating from the consensus sequence are indicated by italics. Transcription of the nfrA transcript starts after a σ^A-dependent promoter. The sequence of a putative PerR box 12 nucleotides upstream of the promoter region is underlined. nt, nucleotides.