TABLE 1.
Characteristics of genes and mutants involved in this study
| Gene | COG/Pfam-predicted general function | Methanol MIC (mM)a | Complementation forb:
|
MDH test result | dH4MPT test resultc | |
|---|---|---|---|---|---|---|
| Growth | C1 sensitivity | |||||
| ORF5 | Cofactor biosynthesis | 0.09 | − | + | + | − |
| ORF7 | Cofactor biosynthesis | 0.13 | + | + | + | + |
| ORF9 | C5 ring cleavage | 0.10 | − | − | + | − |
| ORF17 | Ribonucleotide isomerase | 0.20 | + | + | + | + |
| ORF19 | Unknown | 0.10 | + | + | + | − |
| ORF20 | Cofactor biosynthesis | 0.10 | + | + | + | − |
| ORF21 | Kinase | 0.09 | − | + | − | − |
| ORF22 | Unknown | 0.09 | − | + | Low | − |
a
For comparison, the wild-type strain is not inhibited by 100 mM methanol.
b
+, positive; −, negative. Results of expressing a gene in trans, from a low-activity or a high-activity promoter, are shown.
c
+, dH4MPT detected at or above the wild-type level of 64 μM dH4MPT; −, dH4MPT undetectable (detection limit, 3 μM dH4MPT).