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Normalized relative expression (mean ± SE) of CYP11B1, CYP21A2, and MC2R by ex vivo cultured fetal adrenal fragments (A) and fetal testis fragments (B), cultured for 5 days and exposed for 4 days to 10 µM ACTH (black) or basal media control (gray). Differences in expression between the ACTH-exposed and unexposed condition were tested using a paired t-test (n = 5).
